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From the beginnings of automation in liquid chromatography, the chromatographic data have been monitored as trace of detector signal vs.time.This necessitates excellent stability of flow rate over time.The fixed-flow approach has several advantages: the possibility to immediately integrate signals from concentration-sensitive detectors for quantitation, the immediate availability of retention times as a measure for retention factors, and the possibility to execute programs in different parts of the system in co-ordinated mode based on a single point synchronization..