The stigma is a critical organ in angiosperm for its interaction with pollen grains.It supports compatible pollen germination and tube growth, resulting in fertilization and seed production.Stigmas are mainly divided into two types, dry and wet, according to the absence or presence of exudates on their surfaces.To further investigate the molecular mechanisms underlying stigma functions and differences between dry and wet stigma types, we used 2-D)E and mass spectrometry to identify proteins that are specifically or preferentially expressed in the stigmas of maize (Zea Mays, dry stigma) and tobacco (Nicotiana tabacum, wet stigma), as well as proteins rinsed from the surface of the tobacco stigma.We found that the exclusively expressed proteins in maize and tobacco stigmas were diverse in terms of their sequence but similar with respect to the proportion of each functional category.