论文部分内容阅读
Purpose: Our previous studies found that epidermal stem cells (ESC) proliferation could be promoted when exposed to 50 Hz electromagnetic fields (EMF) in vitro.In addition,we also found that collagen sponge scaffolds can serve as a delivery vehicle for ESC.In order to explore the possible efficacy of EMF for skin tissue engineering,the effects of EMF exposure on ESC transplantaion seeded in collagen sponge scaffolds for wound healing in a murine model were investigated.Materials and methods: The wound models of a full-thickness defect established with 36 7~8-week-old nude mice were randomly divided into three groups: a control group,an ESC-only group,and an ESC with EMF exposure group (frequency of 50 Hz,magnetic induction of 5 mT,60 min per day for 20 d).ESC were separated from human foreskin and cultured in vitro,and then transplanted with collagen sponge scaffolds as a delivery vehicle to wounds of the ESC-only group,and ESC with EMF exposure group was exposed to EMF after ESC transplantaion.The effects of EMF on morphological changes and the expression of β1 integrin in regenerated skins were observed.The wound healing rates and healing times were collected to evaluate the efficacy of repairment.Results: Human ESC were successfully transplanted to nude mice which fascillitated the formation of intact skin on nude mice.In contrast to the other groups,the wound healing of ESC with EMF exposure group was the fastest (P < 0.05),the structure of regenerated skins was more mature,and it contained more continuity in the number of viable cell layers and rich hair follicles structure.Conclusions: The use of 50Hz EMF as a non-invasive treatment can accelerate the wound healing of ESC transplantaion,and restore the structural integrity of regenerated skin.