Chromosome-centric human proteome project(C-HPP)aims at differentiating chromosome-based and tissuespecific protein compositions in terms of protein expression,quantification and modification.We previously found that the analysis of translating mRNA(mRNA attached to ribosome-nascent chain complex,RNC-mRNA)can explain over 94%of mRNA-protein abundance.Therefore,we propose here to use RNC-mRNA information to illustrate protein expression both qualitatively and quantitatively.