It has been demonstrated that silk fibroin(SF)has a significant influence on the crystallization and morphology of CaCO3 in the biomineralization process.However,specific regulation methods and mechanism to get stable biominerals with high application value is rarely researched.Therefore,we use silk fibroin nanoparticles(10-20nm)to explore the influence of mineralization time,concentration of reactants,and uniformity of silk fibroin templates on crystallization behavior of CaCO3.