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本文以酸性铬蓝K为显色剂,在pH10的氨性缓冲溶液中,用双波长吸光光度法,同时测定了钙、镁。采用的波长组合为:测镁时为512～470nm;测钙时为541～528nm。
In this paper, acid chrome blue K as color reagent, pH10 in ammonia buffer solution, using dual-wavelength absorption spectrophotometry, simultaneous determination of calcium and magnesium. The wavelength combination used is: 512 ~ 470nm when measuring magnesium; 541 ~ 528nm when measuring calcium.