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为探讨铝胁迫对大豆根系有机酸累积的影响,以大豆品种粤春03-3为研究对象,通过水培方法进行不同时间的铝处理,分别测定大豆根系的相对生长率、以及距根尖0~2和2~4 cm根段的铝含量、苹果酸浓度、柠檬酸浓度和草酸浓度等。结果表明:铝处理明显抑制了大豆根系的生长。随着铝处理时间的延长,0~2 cm根段的铝含量明显降低,而2~4 cm根段的铝含量明显增加。另外,铝胁迫对不同根段的有机酸累积的影响也不同。铝处理6 h显著提高了大豆根系的苹果酸浓度,但对柠檬酸和草酸浓度均无明显影响。铝处理12 h后,0~2 cm根段中3种有机酸的浓度在铝处理和对照之间均无明显差异,但2~4 cm根段的苹果酸浓度和草酸浓度均明显高于对照。说明苹果酸和草酸在大豆根系铝毒的内部忍耐机制中起着重要作用。
In order to investigate the effect of aluminum stress on the accumulation of organic acids in soybean roots, the soybean variety Yuechun 03-3 was used as the research object, and the relative growth rate of soybean root system was determined by hydroponics at different time, ~ 2 and 2 ~ 4 cm roots aluminum content, malic acid concentration, citric acid concentration and oxalic acid concentration. The results showed that aluminum treatment significantly inhibited the growth of soybean roots. With the extension of aluminum treatment time, the content of aluminum in 0 ~ 2 cm root decreased significantly, while the content of aluminum in 2 ~ 4 cm root increased obviously. In addition, the effects of aluminum stress on the accumulation of organic acids in different roots were also different. Aluminum treatment 6 h significantly increased the malic acid concentration in soybean roots, but had no significant effect on citric acid and oxalic acid concentration. After 12 h of aluminum treatment, the concentrations of three organic acids in 0 ~ 2 cm roots did not show significant difference between aluminum treatment and control, but the concentrations of malic acid and oxalic acid in 2 ~ 4 cm roots were significantly higher than those in control . This indicates that malic acid and oxalic acid play an important role in the internal patience mechanism of soybean root aluminum toxicity.