论文部分内容阅读
大豆疫霉根腐病是大豆的毁灭性病害。为了深入了解大豆对疫霉菌的分子抗病机制,以大豆疫霉菌1号生理小种游动孢子接种抗性品种绥农10的根部及下胚轴,通过反转录差异显示技术分离到疫霉菌侵染0、0.5、1、2和4h后大豆下胚轴和茎部的差异表达基因,其中至少有8个基因与抗病相关。接种后0.5 h开始上调表达的有肉桂酸-4-羟化酶基因、ATP合成酶β亚基基因,以及类花生泛素结合酶基因;接种后1h和2h依次开始上调表达的有尿苷二磷酸-N-乙酰基-α-D-氨基半乳糖基因和豌豆蓝铜蛋白基因;接种后4 h才上调表达的有TGA型碱性亮氨酸拉链基因、大豆环孢素基因和14-3-3蛋白基因。这8个基因中有1个基因与信号传导有关、4个基因与抗病和防御有关、2个基因与转录调控有关、1个基因与能量代谢有关。研究表明,以上8个基因在疫霉菌游动孢子萌发、侵入大豆和在大豆体内扩展过程中起着重要作用。
Phytophthora sojae is a devastating disease of soybeans. In order to further understand the mechanism of molecular resistance to Phytophthora sojae, the roots and hypocotyls of resistant cultivar Suinong 10 were inoculated with zoospore of Phytophthora sojae No.1, and the Phytophthora capsici At 0, 0.5, 1, 2 and 4 h after infection, differentially expressed genes were identified in hypocotyls and stems of at least 8 of them, which were associated with disease resistance. At 0.5 h after inoculation, cinnamic acid-4-hydroxylase gene, ATP synthase β subunit gene and peanut ubiquitin-conjugating enzyme gene were up-regulated at 0.5 h after inoculation, and at 1 h and 2 h after inoculation, N-acetyl-α-D-galactosamine phosphate gene and pea blue copper protein gene; TGA type alkaline leucine zipper gene, soybean ciclosporin gene and 14-3 -3 protein gene. One of the eight genes involved in signal transduction, four genes involved in disease resistance and defense, two genes involved in transcriptional regulation, and one gene related to energy metabolism. Studies have shown that these eight genes play an important role in zoospore germination, invasion of soybean and expansion in soybean.