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利用固相法合成了二十个含羟基氨基酸的小肽。其中，以０．５ｍｏｌ·Ｌ－１二甲二氯硅烷／１．５ｍｏｌ·Ｌ－１苯酚／ＤＣＭ＊为脱除Ｂｏｃ试剂，以ＴＦＭＳＡ为切除树脂试剂。经Ｃ－１８反相柱纯化后，全部产物均通过氨基酸分析要求。体外黄体细胞分泌孕酮实验表明有八个肽化物ＧｌｙＴｙｒＡｌａＬｙｓ，（ＳａｒＳｅｒ）２Ｌｙｓ及其申酯，ＴｙｒＬｙｓ，ＨｉｓＴｙｒ－ＮＨ２，ＴｈｒＰｒｏＴｙｒＬｙｓ－ＮＨ２，ＴｙｒＴｈｒＰｒｏＡｒｇＬｙｓ，ＡｓｐＨｉｓＰｒｏＴｈｒ－ＰｈｅＬｙｓ显示较强的抑制ｈＣＧ致孕酮分泌的活性，而且前三个肽还能显著抑制基础孕酮的分泌，相反，ＧｌｙＳｅｒＴｙｒ能刺激基础孕酮的分泌。目前尚未建立合理的结构一活性关系。
Twenty hydroxyl-terminated amino acids were synthesized by solid-phase method. Among them, the Boc reagent was removed with 0.5 mol·L-1 dichlorodichlorosilane / 1.5 mol·L-1 phenol / DCM *, and TFMSA was used as the excision resin reagent. After purification by C-18 reverse phase column, all products were required for amino acid analysis. In vitro progesterone secretion assay showed that there are eight peptides GlyTyrAlaLys, (SarSer) 2Lys and their esters, TyrLys, HisTyr-NH2, ThrProTyrLys-NH2, TyrThrProArgLys, AspHisProThr-PheLys showed stronger inhibition of hCG-induced progesterone secretion Activity, and the first three peptides also significantly inhibited the secretion of basal progesterone, whereas GlySerTyr stimulated the secretion of basal progesterone. At present, no reasonable structure-activity relationship has been established.