论文部分内容阅读
用人乳头瘤病毒（ＨＰＶ）保守基因的通用引物和１６、１８型特异性引物，通过聚合酶链反应（ＰＣＲ），检测２０例正常新鲜活检食管粘膜，４０例新鲜食管癌组织和５１例石蜡包埋的食管癌组织标本的ＨＰＶＤＮＡ，在２０例正常人食管粘膜组织中ＨＰＶＤＮＡ检出率９．１％，癌组织中为３６．２％，鳞癌和腺癌分别为３４．７％、４７．１％，鳞癌以１６型感染为主，腺癌以１８型为主。但肿瘤分化程度与ＨＰＶ感染无关。结果提示：ＨＰＶ感染与食管癌的发生有一定关系，不同型别的ＨＰＶ与不同组织的亲和性有差别。
Using the common primers for human papillomavirus (HPV) conserved genes and type 16 and 18 specific primers, 20 cases of normal fresh biopsy esophageal mucosa, 40 cases of fresh esophageal cancer tissue, and 51 paraffin cases were detected by polymerase chain reaction (PCR). The HPV DNA of the buried esophageal cancer tissue samples was 9.1% in 20 esophageal mucosal tissues, 36.2% in cancerous tissue, and 34.7% in squamous cell carcinoma and adenocarcinoma, respectively. In 1%, squamous cell carcinoma is mainly infected with type 16 and adenocarcinoma is mainly type 18. However, the degree of tumor differentiation has nothing to do with HPV infection. The results suggest that HPV infection has a certain relationship with the occurrence of esophageal cancer, and the affinity of different types of HPV with different tissues is different.