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人类免疫缺陷病毒1型(HIV-1)主要通过细胞间接触方式传播。作者认为,母乳中存在感染细胞或缺乏抗感染物质或两种因素都存在时,更有助于HIV-1通过母乳传播。作者收集了非洲卢旺达首都吉佳利的215例HIV-1感染产妇产后15天、6个月和18个月的乳汁标本,采用免疫蛋白印迹(Western印迹)技术,测定HIV-1的IgG、SIgA与IgM(后两者先以蛋白G除IgG)。另用PCR技术(取3对引物——gag、pol和env)对产后15天与6个月的母乳标本检测病毒基因组,确定乳汁中感染细胞的存在。婴幼儿是否感染HIV-1依据血清学和临床指征判定。
Human immunodeficiency virus type 1 (HIV-1) is transmitted primarily through cell-cell contacts. The authors believe that the presence of infected cells in breast milk or the absence of anti-infectives or both, contributes to the spread of HIV-1 through breast milk. We collected milk samples from 215 HIV-1 infected mothers at post-natal 15, 6 and 18 months post-Giving birth in Kigali, the capital of Rwanda. Immunoglobulin Western blotting was used to determine the IgG, SIgA And IgM (the latter two with protein G in addition to IgG). Another PCR technique (take three pairs of primers - gag, pol and env) on the 15th and 6th month postpartum breast milk samples to detect the virus genome to determine the presence of infected cells in milk. Whether or not an infant is infected with HIV-1 is based on serological and clinical indications.