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用气相色谱法测定鳀鱼鱼油中ＥＰＡ、ＤＨＡ的含量，鱼油ＰＵＦＡ用０．５ｍｏｌ／ＬＮａＯＨ－甲醇液皂化，ＢＦ＿３－甲醇酯化成脂肪酸甲酯，进行气相色谱测定。内标物用ＴＳＡ－Ｍ。模拟试验５份平均回收率ＥＰＡ－Ｍ为８９．８６％，ＤＨＡ－Ｍ为８９．７８％。变异系数ＥＰＡ－Ｍ为０．８０％，ＤＨＡ－Ｍ为０．６４％。表明本法简便、快速、重现性好。
The contents of EPA and DHA in the fish oil of anchovy were determined by gas chromatography. The PUFA of fish oil was saponified with 0.5mol / L NaOH-methanol solution, and the fatty acid methyl ester of BF_3-methanol was esterified and determined by gas chromatography. Internal standard substance used TSA-M. The average recoveries of five samples in simulated test were 89.86% for EPA-M and 89.78% for DHA-M. The coefficient of variation EPA-M was 0.80%, DHA-M was 0.64%. This method shows that this method is simple, rapid and reproducible.