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目的检测HBsAg阴性献血者HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc及HBVDNA。探讨加强对献血者隐匿性乙型肝炎检出的必要性及输血安全的重要性。方法收集2108例志愿献血的血液样本,酶联免疫吸附法(ELISA)检测其乙型肝炎五项标志物;核酸扩增及检测技术检测HBVDNA。结果 2108例献血者中,共有42份血清呈HBVDNA阳性,检出率达2.08%。其中,HBsAg(+)、HBcAb(+)组占4.35%(1/23);HBsAb(+)、HBeAb(+),HBcAb(+)组占8.16%(8/98);HBsAb(+)组为2.00%(13/651);HBeAg(+)、HBeAb(+)组占8.00%(4/50);HBeAb(+)组占4.67%(5/107);五项血清标志物均为阴性的一组为1.05%(11/1052)。结论联合检测献血者乙型肝炎五项标志物和HBVDNA,有助于提高隐匿性乙型肝炎检出率,并可增加临床输血的安全性。
Objective To detect HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc and HBVDNA in HBsAg-negative donors. To explore the importance of strengthening the detection of occult hepatitis B in blood donors and the safety of blood transfusion. Methods A total of 2108 blood samples were collected for blood donation. Five markers of hepatitis B were detected by enzyme - linked immunosorbent assay (ELISA). HBVDNA was detected by nucleic acid amplification and detection. Results Of 2108 blood donors, a total of 42 serum samples were positive for HBVDNA, with a detection rate of 2.08%. HBsAb (+), HBeAb (+), and HBcAb (+) accounted for 8.16% (8/98) of HBsAb (+) and HBcAb (4/50) in HBeAg (+) group and 4.67% (5/107) in HBeAb (+) group; all five serum markers were negative The group of 1.05% (11/1052). Conclusions Combined detection of five markers of hepatitis B and HBVDNA in blood donors can help increase the detection rate of occult hepatitis B and increase the safety of clinical transfusion.