论文部分内容阅读
目前Lett等建立的染色体姐妹单体交换技术和Goodpasfure建立的染色体硝酸银染色技术在细胞遗传学中已得到广泛运用。人们把姐妹染色单体交换(Sisters chromatid exchange,SCE)的频率看作是反映DNA损伤的敏感指标,并用于对某些疾病的病因研究。人们通过硝酸银染色技术能使染色体的核仁组成区(nucleolusorganizer region,NOR)特异性染成深色,从而探测细胞rRNA基因的活性。所以这两种方法常常被同时用于同一被检者。我们在进行这两项工作时,为了制片和镜检的方便,曾经试将两项技术进行复合显示,其效果比较满意,现介绍于下。
At present, Lett and other established sister exchange of chromosomes and Goodpasfure chromosome silver nitrate staining technology has been widely used in cytogenetics. Sisters chromatid exchange (Sisters chromatid exchange, SCE) frequency as a reflection of DNA damage sensitive indicators, and for the etiology of certain diseases. By means of silver nitrate staining, the nucleolar organizer region (NOR) of a chromosome can be specifically stained dark to detect the activity of a cellular rRNA gene. So these two methods are often used for the same subject at the same time. When we conducted these two tasks, we tried to display the two technologies in a composite manner for the convenience of film making and microscopic examination. The results were satisfactory and are presented below.