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选取妊娠14d的孕鼠20只,采用金属栅栏静式培养法,将腭器官分别培养24和48h,肉眼以及苏木精-伊红染色观察腭器官在体外的发育过程。结果:腭器官发育良好,细胞形态正常。腭器官培养24h后可见到中脊上皮;培养48h后可见中脊上皮消失,腭突融合。结论:本方法为研究腭裂发病机制提供了一个良好的体外模型。
Twenty pregnant rats were selected at 14 days gestation. The palate organ was cultured in the static culture of metal fence for 24 and 48 hours respectively. The development of the palatal organ in vitro was observed with naked eyes and hematoxylin-eosin staining. Results: The palatal organs developed well and the cell morphology was normal. Palate organ culture can be seen after 24h of the midrib epithelium; 48h after the culture disappeared in the ridge epithelium, palatal fossa fusion. Conclusion: This method provides a good in vitro model for studying the pathogenesis of cleft palate.