一、前言 使用酶分析法定量某一种物质,比如在诊断、治疗、畜牧业、农业、水产、科研和环保等方面已有广泛的应用,具有重要意义。 在酶分析中,常先借助酶反应使被测物质定量地进行转变,然后,测定底物、产物或辅酶物质等变化量。特别用辅酶变化量去定量某些物质时,常以某种条件下吸光度具有最大特征吸 I. INTRODUCTION It is of great significance to use enzyme analysis to quantify a certain substance, for example, it has been widely used in diagnosis, treatment, animal husbandry, agriculture, aquatic products, scientific research and environmental protection. In the enzyme analysis, often with the first enzyme reaction so that the measured substance quantitative change, and then determine the substrate, product or coenzyme substances and other changes. In particular, changes in the amount of coenzyme to quantify certain substances, often under certain conditions absorbance has the largest characteristic absorption