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在许多作物中,利用生长点组织培养,已可达到无病毒化并进入大量繁殖的应用阶段。1978年在日本首次发现莲藕病毒病。考虑到莲藕是营养繁殖的作物,因而,将来有必要进行无病毒苗生产或试管优良种苗的大量繁殖。本研究旨在建立一种以莲藕无病毒化植株及其大量增殖方法为基础的生长点组织培养方法。 供试材料为白花莲(Nelumbo nucifera)。切取生育期植株地下茎顶部15—20毫米,用70％酒精和稀释20倍的次氯酸钠(有效氯0.25％)溶液各进行30秒和30分钟的杀菌处理,然后用无菌水洗净。接着,在实体显微镜下取下带有1个叶原基的顶芽或侧芽
In many crops, the use of growth point tissue culture, has been achieved without virus and enter the breeding stage of application. In 1978, the first discovery of lotus root virus disease in Japan. Taking into account the lotus root is a vegetative crop, therefore, in the future it is necessary for the production of virus-free seedlings or test-tube seedlings of large quantities of reproduction. The purpose of this study was to establish a growing point tissue culture method based on the lotus root virus-free plants and their multiplication methods. The tested material is Nelumbo nucifera. Cut the top 15-20 mm of the underground stems of the plants during the growth period and sterilize them with 70% alcohol and a 20-fold dilution of sodium hypochlorite (available chlorine 0.25%) for 30 seconds and 30 minutes each, then rinse with sterile water. Next, the top or side bud with one leaf primordia is removed under a solid microscope