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导电聚合物由于其优越的稳定性和电化学性质,一直是蛋白质芯片敏感膜的研究热点.采用化学氧化聚合法分别制备出氨基和羧基功能化导电聚吡咯共聚物薄膜,通过调节体系单体比例(体积比)来改变导电共聚物的化学结构.采用傅里叶变换红外光谱表征了共聚物的化学组成,利用电化学循环伏安法考察共聚物薄膜的电化学活性变化.在此基础上,采用表面等离子谐振生化分析仪原位考察了牛血清白蛋白(BSA)在共聚物薄膜上的吸附动力学过程.由于共聚物薄膜上的功能基团的种类和含量不同,导致BSA吸附动力学和吸附量的差异.可以明显看出,蛋白质更容易在具有高的氨基密度或低的羧基密度的导电聚吡咯薄膜上进行吸附,随着氨基基团含量的增加,BSA在聚合物薄膜上的吸附量增大.相反,随着羧基基团含量的增大,BSA在共聚物薄膜上的吸附量减小.通过上述方法,可以控制蛋白质在导电聚合物上的吸附行为,进而为构建出更为敏感的、可精确控制的蛋白质芯片奠定基础.
Due to its excellent stability and electrochemical properties, conductive polymers have been the research focus of protein chip sensitive membranes.Amino and carboxyl functionalized polypyrrole copolymer films were prepared by chemical oxidation polymerization respectively, (Volume ratio) to change the chemical structure of the conductive copolymer.The chemical composition of the copolymer was characterized by Fourier transform infrared spectroscopy and the electrochemical activity of the copolymer film was investigated by electrochemical cyclic voltammetry.On the basis of this, The adsorption kinetics of bovine serum albumin (BSA) on the copolymer film was investigated in situ using a surface plasmon resonance biochemical analyzer.Due to the different types and contents of functional groups on the copolymer film, the adsorption kinetics of BSA and The difference in the amount of adsorption.It is clear that the protein is more easily adsorbed on the conductive polypyrrole film with a high density of amino groups or a low density of carboxyl groups.With the increase of the content of amino group, the adsorption of BSA on the polymer film On the contrary, the amount of BSA adsorbed on the copolymer film decreases with the increase of the content of carboxyl groups, and by the above method, it is possible to control The adsorption behavior of proteins on conductive polymers will lay the foundation for the construction of more sensitive and precisely controlled protein chips.