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制备了一种新的基因载体材料——赖氨酸修饰的壳聚糖(CTS-lys)包裹的磁性纳米颗粒.优化制备了CTS-lys原料,红外(IR)和核磁(1H-NMR)检测结果表明壳聚糖的大量氨基被赖氨酸修饰.通过共沉淀方法,制备了赖氨酸修饰的壳聚糖磁性纳米颗粒(CTS-lys-MNPs).利用透射电镜(TEM)、激光粒度分析仪、磁力计(VSM)和X射线衍射(XRD)对CTS-lys-MNPs进行了表征,并通过U293细胞,研究了CTS-lys-MNPs的细胞毒性.结果表明,CTS-lys-MNPs的平均粒径为100nm,具有较好的超顺磁性和较低的细胞毒性;在此基础上,通过凝胶电泳实验观察了CTS-lys-MNPs和DNA的结合情况,并通过单光子发射型计算机断层显像仪(SPECT)研究了CTS-lys-MNPs和DNA的复合物在动物体内跨越血脑屏障的能力.结果表明,CTS-lys-MNPs是一种较好的磁靶向基因载体并能成功地跨越血脑屏障.
A new gene carrier material-lysine modified chitosan (CTS-lys) -coated magnetic nanoparticles was prepared.CTS-lys raw materials, IR and 1H-NMR were optimized. The results showed that a large number of amino groups of chitosan were modified by lysine.The lysine-modified chitosan magnetic nanoparticles (CTS-lys-MNPs) were prepared by coprecipitation method.Transmission electron microscopy (TEM), laser particle size analysis The cytotoxity of CTS-lys-MNPs was studied by U293 cells.The results showed that the average of CTS-lys-MNPs Particle size of 100nm, with better superparamagnetism and lower cytotoxicity; on this basis, the binding of CTS-lys-MNPs and DNA was observed by gel electrophoresis experiments, and by single photon emission computed tomography The ability of CTS-lys-MNPs and DNA complexes to cross the blood-brain barrier in animals was studied by SPECT (SPECT) .The results showed that CTS-lys-MNPs was a good magnetic targeting gene carrier and could successfully Across the blood-brain barrier.