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目的:研究人参皂苷Rb_1和Rg_1对脑缺血和再灌损伤的影响。方法:用可逆性不开颅大鼠大脑中动脉梗塞(MCAO)模型观察人参皂苷Rb_1和Rg_1对梗塞范围(IS),运动障碍(ND)及钙,钾含量的影响。结果:在2-h缺血,Rb_110—40mg·kg~(-1)iv于MCAO前给药减小IS 20％—49％,使ND由5.1减至4.1—2.3,抑制钙积累22％—50％,减少钾丢失18％—37％。MCA再通后给药减小IS12％—35％,使ND由5.2减至4.3—3.3,抑制钙积累10—40,减少钾丢失17％—30％。在永久缺血,Rb_140mg·kg~(-1)iv可减少IS,ND,钙积累和钾丢失。Rg_1对永久和2-h缺血均无效。结论:Rb_1是保护缺血脑的活性成份,对缺血和再灌损伤均有效。
Objective: To study the effects of ginsenoside Rb_1 and Rg_1 on cerebral ischemia and reperfusion injury. Methods: The effects of ginsenosides Rb_1 and Rg_1 on infarct size (IS), dyskinesia (ND), and calcium and potassium contents were observed in a reversible non-cranial rat model of middle cerebral artery infarct (MCAO). RESULTS: After 2-h ischemia, Rb_110-40 mg·kg -1 iv before administration of MCAO reduced IS by 20% - 49%, decreased ND from 5.1 to 4.1 - 2.3, and inhibited calcium accumulation by 22% - 50%, reduce potassium loss 18% - 37%. After MCA recanalization, IS12%-35% was reduced, ND was reduced from 5.2 to 4.3-3.3, calcium accumulation was inhibited by 10-40, and potassium loss was reduced by 17%-30%. In permanent ischemia, Rb_140mg·kg -1 iv can reduce IS, ND, calcium accumulation and potassium loss. Rg_1 was ineffective for both permanent and 2-h ischemia. Conclusion: Rb_1 is an active ingredient that protects ischemic brain and is effective for both ischemia and reperfusion injury.