Objective:To prepare folate liposome complexes of recombinant plasmid pEGFP/SDF-1/KDEL wich contained intrakine SDF-1/KDEL gene and to observe its targeting for breast cancer cells.Methods:Reverse phase evaporation method was employed for preparing folate-liposome complexeses of pEGFP/SDF-1/KDEL,so that preparation condition was optimized;and fluorometric method was taken for testing encapsulation efficiency.Particle diameter of liposome was tested by transmission electron microscopy.Complexeses encapsulated with 0.05-0.25 mg/ml calcein were got and incubated with breast cancer cell line MDA-MB-231 cells for 2 to 12 h,then dissolved them with dimethyl sulfoxide,then detected absorbance with microplate reader.Results:The optimized encapsulation condition are as follows:molecular ratio of lecithin and cholesterol was 3:1,rotary speed was 150 r/min under temperature of 43℃,and encapsulation efficiency reached 81%in this experiment.Average liposome particle diameter was 210 nm;8 h after incubation, the intake of MDA-MB-231 cells to 0.25 mg/ml compounds achieved saturation. Conclusion:The pEGFP/SDF-1/KDEL folic acid liposome complexes prepared has a high encapsulating rate,liposome particle diameters are homogeneous,which is available for targeting for breast cancer cells in vitro. Objective: To prepare folate liposome complexes of recombinant plasmid pEGFP / SDF-1 / KDEL wich contained intrakine SDF-1 / KDEL gene and observe its targeting for breast cancer cells. Methods: Reverse phase evaporation method was employed for preparing folate-liposome complexeses of pEGFP / SDF-1 / KDEL, so that preparation condition was optimized; and fluorometric method was taken for testing encapsulation efficiency. Particle diameter of liposome was tested by transmission electron microscopy. Complex encapsulation with 0.05-0.25 mg / ml calcein were got and incubated with breast cancer cell line MDA-MB-231 cells for 2 to 12 h, then dissolved them with dimethyl sulfoxide, then detected absorbance with microplate reader. Results: The optimized encapsulation conditions are as follows: molecular ratio of lecithin and cholesterol was 3 : 1, rotary speed was 150 r / min under temperature of 43 ° C, and encapsulation efficiency reached 81% in this experiment. Average liposome particle diameter was 210 nm; 8 h after incubation of the intake of MDA-MB-231 cells to 0.25 mg / ml compounds achieved saturation. Conclusion: The pEGFP / SDF-1 / KDEL folic acid liposome complexes prepared has a high encapsulating rate, liposome particle diameters are homogeneous, which is available for targeting for breast cancer cells in vitro.