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目的 探讨转化生长因子 β1 (TGF - β1 )在局灶性脑缺血再灌注中的细胞来源和表达规律。方法 在Wistar大鼠大脑中动脉缺血再灌注模型中 ,用免疫组化法和组织病理学方法观察TGF - β1 在脑缺血再灌流不同时相点的表达及其细胞来源。结果 脑缺血再灌流 6hTGH - β1 表达开始增加 ,与对照组比较有显著差异 ,2 4h达峰值 ,96h仍显著高于对照组。TGF - β1 6h首先在纹状体及丘脑缺血再灌流区表达明显 ,1 2h表达分别增加 2 7%和 1 2 % ,并且可见全脑呈弥散性分布。神经元、星形胶质细胞和小胶质细胞均有表达 ,脉胳膜及血管内皮细胞亦有较强染色。结论 急性脑缺血时TGF - β1 表达上调 ,且随缺血再灌流时间不同而水平不同。神经元、星形胶质细胞和小胶质细胞增有表达。急性脑缺血时TGF - β1 表达增高 ,可能为机体对缺血性脑损伤的一种保护性反应
Objective To investigate the origin and expression of transforming growth factor β1 (TGF - β1) in focal cerebral ischemia / reperfusion. Methods The expression of TGF - β1 at different time points after cerebral ischemia - reperfusion and its cell source were observed by immunohistochemistry and histopathology in the middle cerebral artery occlusion (Wistar) rat model of Wistar rats. Results The expression of TTGH - β1 began to increase after 6 h of cerebral ischemic reperfusion, which was significantly different from that of the control group. The peak value reached the peak at 24 h and still significantly higher at 96 h. TGF - β1 6h was first expressed in the striatum and in the ischemic and reperfused area of the thalamic nucleus. The expression of TGF - β1 increased by 27% and 12% respectively after 12 hours, and the whole brain was diffusely distributed. Neurons, astrocytes and microglial cells were expressed, the vascular membrane and vascular endothelial cells also have strong staining. Conclusion The expression of TGF - β1 is upregulated after acute cerebral ischemia, and the level of TGF - β1 is different with the time of ischemia - reperfusion. Neurons, astrocytes and microglia proliferate. Increased TGF - β1 expression in acute cerebral ischemia may be a protective response to ischemic brain injury