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应用噬菌体研究稻白叶枯病,是近几年发展起来的一项新技术,在日本已广泛应用于病害诊断、预测等方面,但所采用的方法都较繁复。我们于1962年开始探索简易方法,以便适合于目前基层研究单位的设备条件,能广泛开展此项工作。 噬菌体的分离,根据文献介绍,一般采用过滤灭菌法、离心沉淀灭菌法或氯仿灭菌法分离,都需要特定的设备或消耗药品,且操作费时。我们试用不灭菌直接分离法,效果也很好,效率也高,每次可分离大量样品,很适用于田间系统测定灌溉水、土壤、稻叶和种子内噬菌体的消长。依样品的处理方式和步骤分混合法、涂抹法和组织分离法三种。
The application of bacteriophage to rice leaf blight is a new technology developed in recent years. It has been widely used in disease diagnosis and prediction in Japan, but the methods used are complicated. We started in 1962 to explore simple ways to adapt to the current conditions at the grass-roots level and to carry out this work extensively. Phage separation, according to the literature, the general use of filter sterilization, centrifugal precipitation or chloroform sterilization method separation, require specific equipment or consumption of drugs, and the operation time-consuming. We tried non-sterile direct separation method, the effect is good, the efficiency is high, each time a large number of samples can be separated, it is suitable for field systems to determine the growth and decline of irrigation water, soil, rice leaves and seeds. According to sample handling methods and steps of mixed law, smear and tissue separation of three.