论文部分内容阅读
目的 探讨HLA DRB1基因多态性与肺结核 (PTB)的遗传关联性及其与临床表现的关系。方法 采用聚合酶链反应 序列特异性引物 (PCR SSP)方法 ,对 74例PTB患者及 90名正常人的HLA DRB1等位基因进行分型。结果 与对照组相比 ,PTB病例组的DRB1 15等位基因频率显著增高 (34 3%比 17 0 % ,Pc<0 0 5 ,RR =2 91) ,HLA DRB1 12基因频率高 (15 4%比 7 5 % ) ,但统计学上无显著性差异 (Pc>0 0 5 ) ;而DRB1 11基因频率显著低于对照组 (1 4%比 9 9% ,Pc <0 0 5 ,RR =0 12 )。HLA DRB1 15阳性患者中复治病例数和耐药病例数均显著高于HLA DRB1 15阴性组 (P <0 0 5 )。结论 HLA DRB1 15可能是PTB的易感基因 ,DRB1 11可能为保护基因 ,HLA DRB1 15基因与PTB临床特征有一定相关性。
Objective To investigate the genetic association between HLA DRB1 gene polymorphism and pulmonary tuberculosis (PTB) and its relationship with clinical manifestations. Methods HLA DRB1 alleles were genotyped in 74 patients with PTB and 90 normal subjects by polymerase chain reaction sequence specific primers (PCR SSP). Results Compared with the control group, the frequency of DRB1 15 alleles was significantly higher in PTB patients (34.3% vs 17.0%, Pc <0.05, RR = 2 91) (P <0.05), while the frequency of DRB1 11 gene was significantly lower than that of the control group (14% vs 99%, Pc <0.05, RR = 0 12). The number of relapse and resistance cases in HLA DRB1 15 positive patients was significantly higher than that in HLA DRB1 15 negative patients (P <0.05). Conclusion HLA DRB1 15 may be a susceptibility gene of PTB, DRB1 11 may be a protective gene, and HLA DRB1 15 gene may have a certain correlation with clinical features of PTB.