为了检测三磷酸腺苷(ATP)的浓度,通过Au-S键将巯基修饰的三磷酸腺苷的适体ssDNA自组装到薄膜金电极上,制备了一种可灵敏准确地检测ATP浓度的电流型适体传感器。首先研究了薄膜金电极的制备方法,接着研究了三磷酸腺苷的适体ssDNA在薄膜金电极上的组装方法,然后利用循环伏安法研究了自组装适体传感器在K3Fe(CN)6溶液中的响应电流与被测物ATP浓度之间的关系,最后对自组装适体传感器的线性度和重复性进行了分析。实验结果表明:自组装的电流型适体传感器在检测ATP浓度时,传感器的检出限为109 mol/L,线性范围10-9～10-5 mol/L,线性度为0.996 7。自组装的ATP适体传感器检出限低,线性检测范围宽,重复性好。 In order to detect the concentration of adenosine triphosphate (ATP), aptamer ssDNA of thiol-modified adenosine triphosphate (ATP) was self-assembled on the gold electrode by Au-S bond. A current-type aptamer sensor was designed to detect ATP concentration sensitively and accurately. Firstly, the preparation method of thin gold electrode was studied. Then, the assembly method of adenosine triphosphate (ssDNA) on the gold electrode was studied. Then the response of self-assembled aptamer sensor in K3Fe (CN) 6 solution was studied by cyclic voltammetry Current and the measured ATP concentration relationship between the final self-assembled aptamer linearity and repeatability were analyzed. The experimental results show that the detection limit of the sensor is 109 mol / L, the linear range is 10-9 ~ 10-5 mol / L and the linearity is 0.996 7 when self-assembled current-type aptamer is used to detect ATP concentration. Self-assembled ATP aptamer sensor has low detection limit, wide linear detection range and good repeatability.