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目的调查深圳市公共场所中分枝杆菌的污染状况。方法于2012年11月采集商场、宾馆和综合性医院中水、土壤、风管积尘和气溶胶样本,巢式PCR方法扩增结核分枝杆菌复合群IS6110和分枝杆菌Hsp65基因,琼脂糖凝胶电泳检测扩增产物。结果深圳公共场所中水、土壤、风管积尘和气溶胶样本中结核分枝杆菌复合群阳性率分别为4.68%、0.97%、5.41%和3.85%;分枝杆菌阳性率分别为21.1%、4.85%、17.1%和13.1%。结论结核杆菌复合群在宾馆及商场空调积尘、自来水和空调冷却水中阳性率较高,分枝杆菌在空调冷却水、自来水和综合性医院气溶胶及空调积尘中阳性率较高。应引起重视,强化防控措施,保护人群健康。
Objective To investigate the contamination of mycobacteria in public places in Shenzhen. Methods Samples of water, soil, duct dust and aerosol collected from shopping centers, hotels and general hospitals were collected in November 2012. The nested PCR method was used to amplify the Mycobacterium tuberculosis complex IS6110 and mycobacterium Hsp65 gene. The agarose gel Gel electrophoresis detection of amplification products. Results The positive rate of Mycobacterium tuberculosis complex in water, soil, duct dust and aerosol samples in public places in Shenzhen was 4.68%, 0.97%, 5.41% and 3.85%, respectively. The positive rates of mycobacteria were 21.1% and 4.85 %, 17.1% and 13.1%. Conclusion The positive rate of Mycobacterium tuberculosis complex in residential buildings and air-conditioners in shops and shopping malls is high. The positive rate of mycobacteria in air-conditioning cooling water, tap water and general hospital aerosols and air-conditioners is high. Should pay attention to and strengthen prevention and control measures to protect the health of the population.