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目的:观察黄芪注射液(AI)对小鼠胸腺萎缩的干预作用。方法:将昆明小鼠随机分为正常对照组、模型组、IL-2组、黄芪注射液高(2 g/kg)、中(1 g/kg)、低(0.5 g/kg)剂量组,除正常对照组外,均采用地塞米松(25 mg/kg)腹腔注射复制小鼠胸腺淋巴细胞凋亡模型。观察黄芪注射液对小鼠胸腺指数的影响及胸腺的形态学改变,TUNEL及胸腺琼脂糖凝胶电泳测胸腺淋巴细胞凋亡,采用免疫组织化学与医学图像分析系统结合的方法检测小鼠胸腺淋巴细胞相关凋亡基因Bc l-2的表达。结果:黄芪注射液能明显改善萎缩的胸腺,增加胸腺指数(P<0.05);黄芪各剂量组小鼠胸腺皮质细胞明显增多,胸腺淋巴细胞凋亡数目及阳性细胞百分比与模型组比较显著减少(P<0.05或P<0.01),DNA琼脂糖凝胶电泳与模型组相比有显著变化,DNA的梯状图谱显色变浅甚至消失,同时还发现黄芪注射液能提高Bc l-2的表达(P<0.05或P<0.01)。结论:黄芪注射液有明显的抗胸腺萎缩及抑制胸腺淋巴细胞凋亡的作用,其作用机制可能与上调Bc l-2基因表达相关。
Objective: To observe the effect of astragalus injection (AI) on the thymus atrophy in mice. Methods: Kunming mice were randomly divided into normal control group, model group, IL-2 group, Huangqi injection high (2 g/kg), middle (1 g/kg) and low (0.5 g/kg) dose groups. In addition to the normal control group, dexamethasone (25 mg/kg) was intraperitoneally injected to replicate the mouse thymic lymphocyte apoptosis model. Observe the effect of Huangqi injection on thymus index of mice and morphological changes of thymus. TUNEL and thymus agarose gel electrophoresis were used to measure lymphocyte apoptosis of thymus. Immunohistochemistry and medical image analysis system were used to detect thymic lymph node in mice. Cell-associated apoptosis gene Bcl-2 expression. RESULTS: Astragalus membranaceus injection significantly improved atrophic thymus and increased thymus index (P<0.05); Astragalus membranaceus significantly increased the number of thymic cortical cells in mice, and the number of thymocyte lymphocytes apoptosis and percentage of positive cells were significantly reduced compared with the model group ( P<0.05 or P<0.01). The DNA agarose gel electrophoresis showed significant changes compared with the model group. The DNA ladder coloration became lighter or even disappeared. It was also found that Huangqi injection could increase the expression of Bc l-2. (P<0.05 or P<0.01). Conclusion: Astragalus membranaceus injection has obvious anti-thymus atrophy and inhibition of thymocyte apoptosis, and its mechanism may be related to up-regulation of Bcl-2 gene expression.