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目的研究共轭亚油酸(CLA)对泌乳鼠肝脏脂类代谢相关基因mRNA表达的影响。方法选取16只泌乳昆明鼠,随机分为对照组(1.5%亚油酸)和CLA组(1.5%共轭亚油酸),每组8只,从泌乳D日喂至D18,检测母鼠胰岛素耐受,采集血液和肝脏组织,分析血液指标和肝脏脂类代谢相关基因mRNA的表达。结果 CLA组泌乳鼠肝脏重量和甘油三酯含量与对照组比没有显著差异(P>0.05),CLA组泌乳鼠血液中的胆固醇、高密度脂蛋白、低密度脂蛋白和谷氨酰转肽酶的含量均显著高于对照组(P<0.05,P<0.01),CLA组泌乳鼠血液中胰岛素的含量也显著升高,形成胰岛素抵抗。与对照组比,CLA组泌乳鼠肝脏中硬脂酰辅酶A去饱和酶(SCD)基因的mRNA表达显著上调(P 0.05),而脂肪酸合成酶(FASN)、脂肪酸转位酶(CD36)、载脂蛋白B100(APOB100)及调控因子固醇调节元件结合蛋白1c(SREBP1-c)和过氧化物酶体增殖激活受体α(PPARα)基因的mRNA表达均没有显著差异。结论饲料中加入1.5%CLA能够升高泌乳鼠血液中胆固醇、高密度脂蛋白、低密度脂蛋白、谷氨酰转肽酶和胰岛素含量,上调肝脏硬脂酰辅酶A去饱和酶基因mRNA的表达。[营养学报,2012,34(6):531-535]
Objective To investigate the effect of conjugated linoleic acid (CLA) on mRNA expression of lipid metabolism related genes in liver of neonatal rats. Methods Totally 16 lactating Kunming mice were randomly divided into control group (1.5% linoleic acid) and CLA group (1.5% conjugated linoleic acid), with 8 mice in each group. Tolerance, blood and liver tissue acquisition, analysis of blood indicators and liver lipid metabolism related gene mRNA expression. Results There was no significant difference in the liver weight and triglyceride content between the CLA group and the control group (P> 0.05). In the CLA group, blood cholesterol, high density lipoprotein, low density lipoprotein and glutamyl transpeptidase (P <0.05, P <0.01). The content of insulin in lactating blood of CLA mice was significantly increased, forming insulin resistance. Compared with the control group, the mRNA expression of stearoyl-CoA desaturase (SCD) gene in the livers of CLA mice was significantly increased (P <0.05), while the levels of fatty acid synthase (FASN), fatty acid translocase There was no significant difference in the mRNA expression of lipoprotein B100 (APOB100) and regulatory elements sterol regulatory element binding protein 1c (SREBP1-c) and peroxisome proliferator-activated receptor α (PPARα). Conclusion The addition of 1.5% CLA to feed can increase the blood cholesterol, high density lipoprotein, low density lipoprotein, glutamyl transpeptidase and insulin contents, and up-regulate the expression of hepatic stearoyl-CoA desaturase mRNA . [Journal of Nutrition, 2012,34 (6): 531-535]