论文部分内容阅读
目的总结DNA疫苗主要转运途径及其安全性的研究现状,探讨DNA疫苗的不同转运途径在动物实验和人类试验中的应用。方法应用M ed line、PubM ed及CNK I期刊全文数据库检索系统,以“DNA疫苗、电穿孔、基因枪、直接注射、喷雾、脂质体包裹”等为关键词,检索2008-2011年的相关文献,总共检索到英文文献97条。纳入标准:①DNA疫苗的一般特性;②DNA疫苗电传孔转运途径;③DNA疫苗基因枪注射途径;④DNA疫苗直接注射途径;⑤DNA疫苗黏膜免疫途径。根据纳入标准,符合分析的文献33篇。结果 DNA疫苗自偶然被发现以来,显示出了无法比拟的优越性,一举成为第三代疫苗。使用合适的转运途径能增加DNA疫苗在体内的免疫原性和转染效率。然而,转染效率的增加,使得人们对基因整合等安全性问题的担心更甚。结论 DNA疫苗通过合适的转运途径能够增加其在体内的免疫原形和转染效率,各种转运途径均是安全的。在不久的将来,DNA疫苗将能应用于人类,造福世界。但是在实验或临床试验中还是要关注并防止DNA疫苗安全性问题的发生。
OBJECTIVE: To summarize the current research status of DNA vaccine’s main transport routes and its safety, and to explore the application of different DNA vaccine transport routes in animal experiments and human experiments. Methods Using M ed line, PubM ed and CNK I full-text database retrieval system, the key words of “DNA vaccine, electroporation, gene gun, direct injection, spray and liposome encapsulation” Of the relevant literature, a total of retrieved English literature 97. Inclusion criteria: ① the general characteristics of DNA vaccine; ② DNA vaccine teleportation pathway; ③ DNA vaccine gene gun injection; ④ DNA vaccine direct injection; ⑤ DNA vaccine mucosal immunization. According to the inclusion criteria, 33 articles were analyzed. DNA vaccine since the accidental discovery, showing an incomparable superiority, in one fell swoop as a third generation vaccine. Using appropriate transport routes can increase the immunogenicity and transfection efficiency of DNA vaccines in vivo. However, the increased transfection efficiency makes people even more worried about the safety issues such as gene integration. Conclusion The DNA vaccine can increase its immunogen form and transfection efficiency in vivo through appropriate transport routes, and all kinds of transport routes are safe. In the near future, DNA vaccines will be applied to humans for the benefit of the world. However, in experimental or clinical trials or to pay attention to and prevent DNA vaccine safety problems.