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利用吸附法将柱状假丝酵母菌脂肪酶(Candida rugosa lipase,CRL)固定于SBA-15介孔分子筛上,在搅拌槽反应体系中催化拆分外消旋萘普生甲酯的水解反应,获得了光学纯对映体(S)-萘普生,考察了SBA-15性能和酶固定量对初始反应速率、产量、转化率、对映体过剩(eep)和对映体选择性(E)的影响。结果表明,介孔分子筛SBA-15性能对固定化酶催化反应速率有显著影响;反应产量和转化率随酶固定量的增加而线性增大;固定化酶催化反应220 h的转化率达到45.8%,eep达到100%,E200,初始反应速率和对映体选择性都大大高于自由酶的催化性能。
Candida rugosa lipase (CRL) was immobilized on SBA-15 mesoporous molecular sieve by adsorption method, and the hydrolysis of racemic naproxen methyl ester was catalyzed in a stirred tank reaction system to obtain (S) -naproxen, the effect of SBA-15 performance and enzyme immobilization on the initial reaction rate, yield, conversion, enantiomeric excess and enantioselectivity (E) Impact. The results showed that the performance of mesoporous molecular sieve SBA-15 had a significant effect on the catalytic reaction rate of immobilized enzyme. The reaction yield and conversion rate increased linearly with the increase of enzyme immobilization amount. The conversion rate of immobilized enzyme catalyzed reaction for 220 h reached 45.8% , eep reaches 100%, E200, the initial reaction rate and enantioselectivity are significantly higher than the free enzyme catalytic properties.