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利用ＤＮＡ重组技术，将Ⅲ型中国株ＨＣＶＥ２／ＮＳ１基因片段插入真核表达载体，然后转染哺乳动物细胞ＮＩＨ３Ｔ３以表达Ｅ２糖蛋白．检测显示来自３月以上培养的细胞克隆中表达产物分子量为７０ｋＤ，经Ｗｅｓｔｅｒｎｂｌｏｔ证实该表达产物能与抗ＨＣＶ阳性血清进行特异性反应．以上表明首次在哺乳动物细胞中成功表达Ⅲ型中国株ＨＣＶ的Ｅ２糖蛋白，并建立相应的稳定表达细胞系．
The DNA fragment of HCVE2 / NS1 was inserted into the eukaryotic expression vector and transfected into NIH3T3 mammalian cells to express E2 glycoprotein. The results showed that the molecular weight of the expressed product from the cell clones cultured in March was 70 kD. Western blot confirmed that the expressed product could specifically react with anti-HCV positive sera. The above shows that for the first time, E2 glycoprotein of type III Chinese strain HCV is successfully expressed in mammalian cells and a corresponding stable expression cell line is established.