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目的:探讨甲状腺癌细胞中BCORL1对E-cadherin的调控及其对侵袭转移能力的影响。方法:采用Western Blot技术检测人甲状腺癌细胞TA-K、TPC-1和FTC-133中BCORL1及E-cadherin的表达;应用siRNA特异性干扰TA-K细胞中BCORL1的表达,Western Blot和qRT-PCR技术检测BCROL1和E-cadherin表达变化;Transwall侵袭和迁移小室检测BCORL1对TA-K细胞侵袭转移能力的影响。结果:BCORL1在TA-K细胞中的表达水平明显高于TPC-1和FTC-133,而E-cadherin表达水平则明显低于TPC-1和FTC-133;特异siRNA能显著下调BCORL1mRNA(t=14.025,P<0.000 1)和蛋白表达,导致E-cadherin mRNA(t=8.004,P<0.000 1)和蛋白表达升高;下调TA-K细胞中BCORL1表达能显著减弱细胞侵袭和迁移能力(t=5.942,P=0.000 3;t=7.115,P=0.000 1)。结论:人甲状腺癌细胞中BCORL1可能通过转录抑制调控E-cadherin表达,从而促进肿瘤侵袭转移。
Objective: To investigate the effect of BCORL1 on the regulation of E-cadherin and its effect on invasion and metastasis in thyroid cancer cells. Methods: The expressions of BCORL1 and E-cadherin in TA-K, TPC-1 and FTC-133 were detected by Western Blot. The expression of BCORL1 in TA-K cells was detected by siRNA. Western Blot and qRT- PCR technique was used to detect the expression of BCROL1 and E-cadherin. Transwall invasion and migration chambers were used to detect the effect of BCORL1 on the invasion and metastasis of TA-K cells. Results: The expression level of BCORL1 in TA-K cells was significantly higher than that in TPC-1 and FTC-133, while the expression of E-cadherin was significantly lower than that in TPC-1 and FTC-133. (T = 8.004, P <0.0001) and protein expression, and downregulation of BCORL1 expression in TA-K cells significantly reduced the ability of cell invasion and migration (t = 5.942, P = 0.0003; t = 7.115, P = 0.000 1). CONCLUSION: BCORL1 may regulate the expression of E-cadherin via transcriptional repression and promote tumor invasion and metastasis.