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我室在动脉粥样硬化发病机理研究中,要求突出构成动脉壁中平滑肌和胶原纤维二种成分。但国内,外在显示胶原纤维或显示平滑肌的特殊染色中,一直没有特异性的染色法,多为复合染色,而且以显示其它成分为主。往往顾此失彼,不易得到清晰,染色鲜艳,突出平滑肌和胶原纤维成分。为了科研工作的需要,经反复试验,创造出了显示平滑肌和胶原纤维的双色染色法。一、试剂配制 1.丽春红3R(Poncean 3R)液:0.5gm丽春红3R和0.5gm磷钨酸(Phoshotungstic acid)
My room in the study of the pathogenesis of atherosclerosis, requiring the prominent composition of the arterial wall smooth muscle and collagen fibers in two components. However, there are no specific staining methods for the special staining of collagen fibers or smooth muscle in China or abroad. They are mostly complex dyes and show other components. Often careless, not easy to get clear, brightly colored, highlighting the smooth muscle and collagen fibers. In order to research the needs of the work, after repeated experiments to create a two-color dyeing method to show smooth muscle and collagen fibers. I. Reagent Preparation 1. Poncean 3R solution: 0.5 gm Ponceau 3R and 0.5 gm Phoshotungstic acid