目的建立一种检测基孔肯雅病毒纳米金实时荧光定量PCR的方法。方法以课题组建立的普通实时荧光PCR方法为基础,在PCR体系中添加不同大小粒径的纳米金进行体系优化,评价优化后的体系。结果添加纳米金的实时荧光PCR较不添加的扩增效率高;优化后的纳米金实时荧光PCR产物的Ct值与模板稀释浓度存在良好的线性关系,回归方程:y=-3.31x+41.78,R2=0.9997,PCR扩增效率为99.5%。结论纳米金能提高实时荧光PCR的反应效率。 Objective To establish a real-time fluorescence quantitative PCR for detection of Chikungunya virus. Methods Based on the common real-time fluorescence PCR method set up by our research group, we optimized the system by adding nano gold with different size and size to PCR system. Results Compared with the non-added real-time PCR, the amplification efficiency of gold nanoparticles was higher than that of the non-added gold nanoparticles. There was a good linear relationship between the Ct values ​​and the concentration of template dilution. The regression equation was y = -3.31x + 41.78, R2 = 0.9997, PCR amplification efficiency of 99.5%. Conclusion Nanogold can improve the reaction efficiency of real-time fluorescence PCR.