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目的探讨沙利度胺、孕三烯酮及两者联合用药对大鼠子宫内膜异位组织中血管生成的影响。方法24只子宫内膜异位症模型SD大鼠,随机分为模型对照、沙利度胺(20mg·kg-1·d-1)、孕三烯酮(0.5mg·kg-1·d-1)及联合用药(沙利度胺20mg·kg-1·d-1和孕三烯酮0.5mg·kg-1·d-1)4组,每组6只。药物溶于生理盐水中腹腔注射给药,模型对照组每日腹腔注射生理盐水2mL。给药4wk后处死,免疫组织化学SP法测定血管内皮生长因子(VEGF)和肿瘤坏死因子α(TNF-α)在异位内膜的表达,并通过Ⅷ因子标记异位子宫内膜血管,检测异位内膜组织中微血管密度(MVD)。结果沙利度胺组、孕三烯酮组及联合用药组异位内膜MVD、VEGF和TNF-α的表达均显著低于模型对照组(P<0.01),其中联合用药组MVD和VEGF比沙利度胺组和孕三烯酮组降低得更为显著(P<0.05),联合用药组TNF-α与孕三烯酮组无显著差异(P>0.05)。结论沙利度胺和孕三烯酮可抑制大鼠异位内膜的MVD、VEGF和TNF-α的表达,从而抑制子宫内膜异位症血管生成,当两者联合用药时,作用更强。
Objective To investigate the effects of thalidomide, gestrinone and their combination on angiogenesis in rat endometriosis. Methods Twenty-four endometriosis SD rats were randomly divided into three groups: model control, thalidomide (20 mg · kg -1 · d -1), gestrinone (0.5 mg · kg -1 · d -1) 1) and combination therapy (thalidomide 20 mg · kg-1 · d-1 and gestrinone 0.5 mg · kg-1 · d-1). The drug was dissolved in saline for intraperitoneal injection, and the model control group was intraperitoneally injected with 2 mL of normal saline. The rats were killed 4 weeks later, the expression of vascular endothelial growth factor (VEGF) and tumor necrosis factor α (TNF-α) in ectopic endometrium were detected by immunohistochemical SP method, and the ectopic endometrial blood vessels were detected by Ⅷ factor Ectopic endometrial microvessel density (MVD). Results The expressions of MVD, VEGF and TNF-α in the eutopic endometrium of thalidomide group, gestrinone group and combined treatment group were significantly lower than those in the model control group (P <0.01) There was no significant difference between the combination group and the gestrinone group (P> 0.05). Conclusions Thalidomide and Gestrinone can inhibit the expression of MVD, VEGF and TNF-α in ectopic endometrium of rats, thereby inhibiting the angiogenesis of endometriosis. When the combination of the two drugs is more effective .