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OBJECTIVE To study the expression of the nuclear factor kappa B (NF-kappa B) in non-small cell lung cancer (NSCLC),to explore the apoptotic ratio in NSCLC related to different NF-kappa Bs,and to understand the clinical significance of NF-kappa B in NSCLC apoptosis. METHODS NF-kappa B expression in 45 new samples of NSCLC,collected during a period from October to December,2005,was assayed using Western blots,and the apoptotic ratio of NSCLC was determined by the Tunel method. RESULTS Of the 45 patients,the average relative expression of NF-kappa B was 0.6047±0.3572.The expression of NF-kappa B was higher in the poorly differentiated lung cancer cells than in the well-differentiated tumors(P<0.05).The apoptotic ratio was 56.4% in the lung cancer cells with higher NF-kappa B expression,and was 76.7% in those with lower NF-kappa B expression(P<0.05). CONCLUSION The expression of NF-kappa B is correlated with the differentiation of NSCLC.NF-kappa B inhibits apoptosis in NSCLC.As a transcription factor,NF-kappa B plays a very important role both in formation and in development of NSCLC. NF-kappa B might serve as a target for NSCLC gene therapy.
OBJECTIVE To study the expression of the nuclear factor kappa B (NF-kappa B) in non-small cell lung cancer (NSCLC), to explore the apoptotic ratio in NSCLC related to different NF-kappa Bs, and to understand the clinical significance of NF-kappa B in NSCLC apoptosis. METHODS NF-kappa B expression in 45 new samples of NSCLC, collected during a period from October to December, 2005, was assayed using Western blots, and the apoptotic ratio of NSCLC was determined by the Tunel method RESULTS Of the 45 patients, the average relative expression of NF-kappa B was 0.6047 ± 0.3572. The expression of NF-κappa B was higher in the poorly differentiated lung cancer cells than in well-differentiated tumors (P <0.05). The apoptotic ratio was 56.4% in the lung cancer cells with higher NF-kappa B expression, and was 76.7% of those with lower NF-kappa B expression (P <0.05). CONCLUSION The expression of NF-kappa B is correlated with the differentiation of NSCLC.NF-kappa B inhibits apoptosis in NSCLC. As a transcri ption factor, NF-kappa B plays a very important role both in formation and in development of NSCLC. NF-kappa B might serve as a target for NSCLC gene therapy.