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目的了解ASPP1和ASPP2基因5′端非编码区CpG岛在野生型p53肿瘤细胞中的甲基化状况及其与mRNA异常表达的关系。方法用RT-PCR方法测定mRNA表达,甲基化特异的PCR方法测定DNA甲基化。结果肿瘤细胞中ASPP1和ASPP2mRNA表达减少,同时ASPP基因的5′端非编码区出现异常高甲基化。结论ASPP基因的异常高甲基化可能是引起ASPP mRNA表达降低的影响因素之一。
OBJECTIVE: To investigate the methylation status of CpG island with 5 ’non-coding region of ASPP1 and ASPP2 gene in wild type p53 tumor cells and its relationship with abnormal mRNA expression. Methods mRNA expression was determined by RT-PCR and methylation-specific PCR was used to detect DNA methylation. As a result, the expression of ASPP1 and ASPP2 mRNA in tumor cells decreased and the hypermethylation of ASPP gene at the 5 ’non-coding region was abnormal. Conclusion Abnormal hypermethylation of ASPP gene may be one of the factors that cause the decrease of ASPP mRNA expression.