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目的通过对广州地区登革热媒介白纹伊蚊进行携带登革热病毒情况的调查,从传播媒介的角度探索该地区登革热流行的来源和特点。方法采集广州市各区新旧疫点附近的白纹伊蚊幼虫标本,饲养为成蚊后提取蚊虫总RNA,用登革热病毒特异引物经实时荧光逆转录聚合酶链反应(RT-PCR)进行病毒核酸检测,并对阳性结果反应产物进行序列测定。结果在荔湾区逢源街(2006年8月)、从化市邓村(2007年4月)和白云区云溪(2007年5月)各检出1宗登革热病毒核酸阳性蚊虫标本。其中荔湾区逢源街和从化邓村标本的RT-PCR产物经过序列测定证实为登革热病毒Ⅰ型。结论广州地区登革热病毒可能在自然界白纹伊蚊种群中长期存在。
Objective To investigate the origin and characteristics of dengue fever epidemic in the area from the perspective of mass media by investigating the dengue virus carrying dengue vector Aedes albopictus in Guangzhou. Methods Aedes albopictus larvae were collected from old and new epidemic areas in Guangzhou. Total mosquitoes were collected from adult mosquitoes. Virus nucleic acid was detected by RT-PCR with dengue virus specific primers , And the positive reaction products were sequenced. Results One Dengue virus nucleic acid positive mosquito was detected in Feng Yuan Street (August 2006) in Liwan District, Dengcun (April 2007) in Conghua City and Yunxi (Baiyun District in May 2007). Among them, the RT-PCR products of Fengyuan Street and Conghua Dengcun in Liwan District were confirmed as dengue virus type Ⅰ by sequencing. Conclusion Dengue virus in Guangzhou may be long-term in Ae. Albopictus population in nature.