Maize is one of the major crops in China, but maize stalk rot occurs nationwide and has become one of the major challenges in maize production in China. In order to find an environment-friendly and feasible technology to control this disease, a Trichoderma-based biocontrol agent was selected. Forty-eight strains with various inhibition activities to Fusarium graminearum, and Fusarium verticillioides were tested. A group of Trichoderma strains (DLY31, SG3403, DLY1303 and GDFS1009) were found to provide an inhibition rate to pathogen growth in vitro of over 70％. These strains also prevented pathogen infection over 65％ and promoted the maize seedling growth for the main root in vivo by over 50％. Due to its advantage in antifungal activity against pathogens and promotion activity to maize, Trichoderma asperellum GDSF1009 was selected as the most promising strain of the biocontrol agent in the Trichoderma spectrum. Pot experiments showed that the Trichoderma agent at 2–3 g/pot could achieve the best control of seedling stalk rot and promotion of maize seedling growth. In the field experiments, 8–10 g/hole was able to achieve over 65％ control to stalk rot, and yield increased by 2–11％. In the case of natural morbidity, the control efficiency ranged from 27.23 to 48.84％, and the rate of yield increase reached 11.70％, with a dosage of Trichoderma granules at 75 kg ha–1. Based on these results, we concluded that the Trichoderma agent is a promising biocontrol approach to stalk rot in maize.