抗肿瘤药物敏感性测定技术的发展已成为癌症研究的重要问题。由Hamburger 和Salmon 发展的双层软琼脂方法已广泛地用于药物敏感性测定并有很多报道证实其预示准确度。 The development of antineoplastic drug sensitivity assays has become an important issue in cancer research. The double-layer soft agar developed by Hamburger and Salmon has been used extensively for drug susceptibility assays and many reports confirm its predictive accuracy.