论文部分内容阅读
目的检测白细胞介素-8受体A型(CXCR1)和白细胞介素-8受体B型(CXCR2)在强直性脊柱炎(AS)患者外周血中性粒细胞、CD14~+单核细胞和CD3~+T细胞上的表达水平,探讨其与AS疾病活动的相关性和可能涉及的AS炎症发病机制。方法研究对象包括30例活动期AS患者,30例活动期类风湿关节炎(RA)患者和30名健康对照者,应用流式细胞术(FCM)检测CXCR1、CXCR2分别在AS患者、RA患者和健康对照者外周血中性粒细胞、CD14~+单核细胞和CD3~+T细胞上平均荧光强度(MFI)的表达水平,并和AS患者的临床BASFI、BASDAI、红细胞沉降率(ESR)、血清C反应蛋白(CRP)等指标进行相关分析。结果CXCR1在AS患者组外周血CD3~+T细胞上MFI表达水平(41±24)分别较RA患者组(18±10)和健康对照组(19±7)高(P均<0.01)。CXCR2在AS患者组外周血CD14~+单核细胞MFI表达水平(210±54)较健康对照组(300±52)低(P<0.01),与RA患者组(191±53)比较差异无统计学意义(P>0.05);CXCR2在AS患者外周血CD14~+单核细胞上MFI表达下降与患者毕氏疾病功能指数(BASFI)(r=-0.394,P=0.031)、毕氏AS疾病活动指数(BASDAI)(r_s=-0.378,P=0.040)、ESR(r_s=-0.465,P=0.010)、CRP(r_s=-0.648,P=0.000)存在着负相关关系。结论CXCR1和CXCR2分别在AS患者外周血CD3~+T细胞和CD14~+单核细胞表达异常,提示它们可能参与了AS的发病过程。检测AS患者外周血CD14~+单核细胞的MFI表达水平可能是评价AS疾病活动性有价值的潜在的生物学标志之一。
Objective To detect the expression of CXCR1 and CXCR2 in peripheral blood neutrophils and CD14 + monocytes in patients with ankylosing spondylitis (AS) CD3 ~ + T cells on the level of their association with AS disease activity and possible involvement of the pathogenesis of AS inflammation. Methods Thirty active AS patients, 30 active rheumatoid arthritis (RA) patients and 30 healthy controls were included in this study. Flow cytometry (FCM) was used to detect CXCR1 and CXCR2 in patients with AS, RA and The mean fluorescence intensity (MFI) of peripheral blood neutrophils, CD14 ~ + monocytes and CD3 ~ + T cells in healthy controls were compared with those of AS patients, including clinical BASFI, BASDAI, erythrocyte sedimentation rate (ESR) Serum C-reactive protein (CRP) and other indicators were analyzed. Results The MFI expression level of CXCR1 on peripheral blood CD3 ~ + T cells in AS patients was significantly higher than that in RA patients (18 ± 10) and healthy controls (19 ± 7) (41 ± 24), respectively (all P <0.01). The expression of MFI in CD14 ~ + monocytes in peripheral blood of CXCR2 patients (210 ± 54) was lower than that of healthy controls (300 ± 52) (P <0.01), but not significantly different from that of RA patients (191 ± 53) (P> 0.05). The expression of CXCR2 on peripheral blood CD14 ~ + monocytes in patients with CXCR2 was significantly lower than that in patients with Peiji’s disease index (BASFI) (r = -0.394, P = 0.031) There was a negative correlation between BASDAI (r_s = -0.378, P = 0.040), ESR (r_s = -0.465, P = 0.010) and CRP (r_s = -0.648, P = 0.000). Conclusion The abnormal expressions of CXCR1 and CXCR2 in peripheral blood CD3 ~ + T cells and CD14 ~ + monocytes in AS patients suggest that they may be involved in the pathogenesis of AS. Detection of peripheral blood CD14 ~ + monocyte MFI expression levels in patients with AS may be one of the potential biological markers to evaluate the activity of AS disease.