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目的:比较茵陈蒿汤复方合煎配方颗粒(合煎),单味分煎配方颗粒(分煎)和传统煎煮汤剂对四氯化碳(CCl4)造成小鼠肝损伤模型的影响。方法:90只小鼠随机分为空白组、模型组、联苯双酯滴丸组、合煎高、中、低剂量组(17.16,8.58,4.29 g·kg~(-1)),分煎高、中剂量组(17.16,8.58 g·kg~(-1)),传统汤剂组(4.29 g·k~(-1)),共9组。除空白组和模型组每日灌胃蒸馏水,其余7组预防性给药7 d。第7天灌胃结束后,除空白组外,其余各组腹腔注射0.05%CCl4,建立急性肝损伤模型。18 h后,眼眶取血测定各组小鼠血清丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),总蛋白(TP),白蛋白(ALB)和总胆红素(TBIL)活性。取小鼠肝脏做苏木素-伊红(HE)染色观察肝脏病理组织学变化,并取肝脏匀浆测定超氧化物歧化酶(SOD),丙二醛(MDA)水平,简单对比3种不同方法制备茵陈蒿汤中主要有效成分的变化。结果:合煎高、中、低剂量,分煎高、中剂量、传统汤剂均能降低CCl_4致肝损伤程度。合煎高剂量组效果优于高、中剂量组和传统汤剂组(P<0.05,P<0.01)。结论:茵陈蒿汤对CCl_4致小鼠急性肝损具保护作用,合煎配方颗粒可能由于合煎过程中的各有效成分溶出率不同,效果较单味分煎配方颗粒好;与传统汤剂比较,由于煎煮工艺优化有效成分含量更高,效果更好。其保护肝脏机制可能与清除自由基,抑制脂质过氧化有关。
OBJECTIVE: To compare the effects of Yinchuanhao Decoction Compound Prescription Decoction (JHJ), Shanwei Fenjian Granules (JJU) and traditional decoction decoction on liver injury induced by carbon tetrachloride (CCl4) in mice. Methods: Ninety mice were randomly divided into blank group, model group, bifendate dropping pills group, combined with high, medium and low dose fried group (17.16, 8.58, 4.29 g · kg -1) High and medium dose groups (17.16, 8.58 g · kg -1) and traditional decoction group (4.29 g · k -1) groups. In addition to the blank group and model group were fed with distilled water daily, the remaining seven groups prophylactic administration of 7 d. On the 7th day after gavage, except the blank group, the other groups were intraperitoneally injected with 0.05% CCl4 to establish acute liver injury model. Eighteen hours later, the orbital blood was taken to measure the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein (TP), albumin (ALB) and total bilirubin (TBIL) activity. The liver of the mice was subjected to hematoxylin and eosin (HE) staining to observe the histopathological changes of the liver. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in the liver homogenates were measured and compared with three different methods Yinchenhao decoction of the main active ingredient changes. Results: Combined with high, medium and low dose, sub-fried high, medium dose, traditional decoction can reduce CCl 4-induced liver injury. The combined effect of high-dose and high-dose group was better than that of high-dose and middle-dose group and traditional decoction group (P <0.05, P <0.01). CONCLUSION: Yinchenhao decoction can protect acute liver injury induced by CCl_4 in mice. The combined decoction granules may be different from the decoction granules because of the different dissolution rates of the active ingredients in the combined decoction. Compared with traditional decoction In comparison, the effect is better due to the higher content of active ingredients in the decoction process. Its mechanism of protecting the liver may be related to scavenging free radicals and inhibiting lipid peroxidation.