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本研究建立了抗黄曲霉毒素AFM1 的单克隆抗体细胞株。用AFM1-肟-牛血清白蛋白(AFM1 -oxim e-BSA)偶联物免疫Ba1b/c小鼠后,取脾细胞与小鼠骨髓瘤细胞系Sp2/0融合,经3～4次亚克隆建立了3个稳定分泌抗黄曲霉毒素M1 抗体的杂交瘤细胞株,分别命名为3G2,3G3,6G8。3株抗体均属IgG1 亚类。培养上清液的稀释度为1v2 000～1v12 800,腹水的抗体稀释度为1v3.2×106 ～1v20×107,参考工作稀释度为1v1×106 ～1v2.5×106。3G3和6G8抗体的IgG 含量分别为0.8和1.43 g/L,亲和常数分别为5.438和4.369。6G8抗体与其它类型黄曲霉毒素均无交叉反应
In this study, a monoclonal antibody against aflatoxin AFM1 was established. After immunizing Ba1b / c mice with the AFM1-oxim e-BSA conjugate, the spleen cells were fused with the mouse myeloma cell line Sp2 / 0 and subcloned 3 to 4 times Three hybridoma cell lines stably secreting anti-aflatoxin M1 antibody were established and named as 3G2, 3G3 and 6G8.3 respectively. The culture supernatant dilution 1v2 000 ~ 1v12 800, ascites antibody dilution 1v3.2 × 106 ~ 1v20 × 107, the reference working dilution 1v1 × 106 ~ 1v2.5 × 106.3G3 and 6G8 antibody The IgG contents were 0.8 and 1.43 g / L, respectively, and the affinity constants were 5.438 and 4.369.6 G8 respectively. There was no cross-reaction with other aflatoxins