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Superparamagnetic poly(styrene)-co-poly(2-acrylanmido-2-methyl propanesulfonic acid) (PSt-co-PAMPS) and poly(methylmethacrylate)-co-poly(glycidyl methacrylate) (PMMA-co-PGMA) microspheres with mean size of 170 nm were prepared by emulsion polymerization in the presence of oleic acid-coated Fe3O4 nanoparticles. The structures, morphologies, diameter and diameter distribution of the as-prepared microspheres were identified by Fourier transform infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM). The saturation magnetizations of PSt-co-PAMPS and PMMA-co-PGMA microspheres are 21.94 and 25.07 emu/g, respectively. The as-synthesized magnetic microspheres were used for immobilization of Bovine serum albumin (BSA) by physical interaction and covalent interaction respectively. The equilibrium amount of BSA immobilized onto PMMA-co-PGMA microspheres was 86.48 mg/g microspheres in 90 min, while on PSt-co-PAMPS microspheres was 59.62 mg/g microspheres in 120 min.
Superparamagnetic poly (styrene) -co-poly (2-acrylanmido-2-methyl propanesulfonic acid) (PSt-co-PAMPS) and poly (methylmethacrylate) -co-polyglycidyl methacrylate (PMMA- size of 170 nm were prepared by emulsion polymerization in the presence of oleic acid-coated Fe3O4 nanoparticles. The structures, morphologies, diameter and diameter distribution of the as-prepared microspheres were identified by Fourier transform infrared spectroscopy (FT-IR) and transmission electron The saturation magnetization of PSt-co-PAMPS and PMMA-co-PGMA microspheres were 21.94 and 25.07 emu / g, respectively. The as-synthesized magnetic microspheres were used for immobilization of Bovine serum albumin (BSA) by physical The equilibrium amount of BSA immobilized onto PMMA-co-PGMA microspheres was 86.48 mg / g microspheres in 90 min, while on PSt-co-PAMPS microspheres was 59.62 mg / g microspheres in 120 min.