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以白花芥蓝为材料,采用反转录PCR技术克隆得到2个八氢番茄红素脱氢酶基因BaPDS1和BaPDS2,GenBank登录号为KX426039和KX426040,其开放阅读框分别为1 692和1 698 bp,分别编码563和565个氨基酸。同源性及进化树构建结果表明PDS蛋白在进化过程中比较保守,芥蓝PDS基因与甘蓝、白菜、花椰菜等十字花科蔬菜亲缘关系较近。半定量PCR分析发现,BaPDS1和BaPDS2表达水平在芥蓝不同发育时期和组织器官间均存在显著差异。萌动种子期BaPDSs表达量较低,之后迅速上升;BaPDS1在成熟期器官间呈现组成型表达,BaPDS2在器官间表达差异较大,幼果中未检出;花器官中萼片的BaPDSs表达量显著低于其他组织,在开花过程中,雄蕊和雌蕊的BaPDS1表达水平出现上调。
Two white phytoene desaturase genes, BaPDS1 and BaPDS2, were cloned by reverse transcription polymerase chain reaction (PCR). GenBank accession numbers were KX426039 and KX426040, and their open reading frames were 1 692 and 1 698 bp, respectively , Encoding 563 and 565 amino acids, respectively. Homology and phylogenetic tree construction showed that PDS protein was relatively conservative during evolution. Kashgar PDS gene was closely related to cruciferous vegetables such as cabbage, cabbage and cauliflower. Semi-quantitative PCR analysis showed that the expression levels of BaPDS1 and BaPDS2 were significantly different between kale at different developmental stages and tissues and organs. The expression of BaPDSs in the germinating seed stage was low and then rapidly increased. BaPDS1 showed constitutive expression in mature organs and BaPDS2 in organs, but not in young fruits. The expression of BaPDSs in floral organs was significantly lower In other tissues, the expression level of BaPDS1 in stamens and pistils is up-regulated during flowering.