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在评价高效色谱的柱效能时,非保留时间(t_0)的准确测定至为重要.在正相液体色谱中,有人以四氯化碳为t_0物质,看来有问题,有人则建议用比移动相含有较低碳原子的同系物,但其合理性未见验证.我们在5
Accurate determination of the non-retention time (t_0) is of paramount importance in the evaluation of the efficiency of a column for high performance chromatography. It appears that there is a problem with carbon tetrachloride as t_0 in normal phase liquid chromatography. Phase contains lower homologues of carbon atoms, but its rationality has not been verified