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目的监测青海省2007～2008年度季节性流感病毒型与亚型分布,了解2007～2008年度部分A/H3N2分离株血凝素(HA)基因变异情况。方法采集监测哨点医院流感样病例的鼻咽双拭子标本,接种MDCK细胞,采用标准抗血清鉴定阳性分离株型与亚型,并对2007～2008年度部分H3亚型流感病毒进行HA基因测序,分析HA基因变异情况。结果 2007～2008年度在哨点医院送检标本中共分离到144株流感病毒,其中124株为A/H3N2,B型20株,型与亚型有明显分布强弱特征。H3亚型HA序列分析,青海省6株分离株与2008、2009年部分省份分离株及江西同期分离株接近,与WHO2008～2009年度疫苗推荐株相近。结论 WHO推荐的2006～2007年度疫苗株A/Hiroshima/52/2005滞后于在我省流行的流感病毒毒株。
Objective To monitor the distribution of seasonal influenza virus type and subtype in Qinghai Province from 2007 to 2008 and to understand the genetic variation of hemagglutinin (HA) gene in some A / H3N2 isolates from 2007 to 2008. Methods Nasopharyngeal double-swab specimens were collected from sentinel hospital for influenza-like illness. MDCK cells were inoculated and positive isolates and subtypes were identified by standard antiserum. HA gene sequencing was performed on some H3 subtype influenza viruses from 2007 to 2008 , Analysis of HA gene variation. Results A total of 144 influenza viruses were isolated from sentinel hospitals in 2007-2008. Among them, 124 strains were A / H3N2 and 20 were B strains. The distribution of type and subtype was obvious. For H3 subtype HA sequence analysis, 6 isolates of Qinghai province were close to those of isolates from other provinces in 2008 and 2009 and Jiangxi isolates in the same period, similar to WHO vaccine recommended strains from 2008 to 2009. Conclusions The vaccine strain A / Hiroshima / 52/2005 recommended by the WHO from 2006 to 2007 lags behind those strains of influenza virus circulating in our province.