利用PCR技术对细菌DNA基因进行扩增和序列分析,通过多酶切、混合酶切和直接PCR技术对MEOR菌种进行基因跟踪检测,来准确地认识和评价目的菌种的油藏适应性和矿场应用潜力,并确切地说明影响和制约MEOR矿场试验各个环节的主要技术因素,为决策MEOR工艺、指导矿场试验进程,提供重要 The bacterial DNA genes were amplified and sequenced by PCR, and the gene tracing of MEOR was detected by multiple enzyme digestion, mixed enzyme digestion and direct PCR to accurately recognize and evaluate the reservoir adaptability of the target strains and The potential of the mine application and the exact description of the major technical factors that influence and constrain the MEOR field test are important in determining the MEOR process and in guiding the test process in the mine