论文部分内容阅读
目的探讨垂体腺癌组织的超微结构及其血管内皮生长因子(VEGF)和基质金属蛋白酶9(MMP-9)的表达意义。方法选取2007年1月至2014年1月间收治的116例垂体腺瘤患者。采用免疫组化染色法和逆转录聚合酶链式反应检测垂体腺瘤组织中VEGF和MMP-9的表达情况,电子显微镜下观察垂体腺瘤的超微结构,并结合VEGF和MMP-9的表达对垂体腺瘤患者的侵袭性进行分析。结果电镜下,侵袭性垂体腺瘤可见较多的粗面内质网、形态怪异的胞核、丰富的核糖体及较大的核仁;非侵袭性垂体腺瘤的核异形率、核仁出现率、线粒体数目和核浆比例均低于侵袭性垂体腺瘤,差异有统计学意义(P<0.05)。非侵袭性垂体腺瘤患者的VEGF、MMP-9蛋白和mRNA表达均低于侵袭性垂体腺瘤患者,差异有统计学意义(P<0.05)。侵袭性垂体腺瘤组患者的VEGF和MMP-9蛋白表达水平无显著差异,但在mRNA水平上呈正相关(r=0.463,P<0.05)。结论电镜观察结果和免疫组化结果均显示侵袭性垂体腺瘤患者的超微结构和侵袭性生长有一定的相关性,且VEGF和MMP-9与新生血管的形成有一定的联系。
Objective To investigate the ultrastructure of pituitary adenocarcinoma and its significance of expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase 9 (MMP-9). Methods One hundred and sixteen patients with pituitary adenomas admitted between January 2007 and January 2014 were enrolled. The expression of VEGF and MMP-9 in pituitary adenomas was detected by immunohistochemical staining and reverse transcriptase-polymerase chain reaction. The ultrastructure of pituitary adenomas was observed by electron microscopy. The expressions of VEGF and MMP-9 The invasiveness of pituitary adenomas was analyzed. Results Electron microscopy revealed more rough endoplasmic reticulum, abnormally shaped nuclei, abundant ribosomes and larger nucleoli in the invasive pituitary adenomas. The nucleus of noninvasive pituitary adenomas showed abnormal appearance of nucleoli The rates of mitochondria and cytoplasm were lower than those of invasive pituitary adenomas (P <0.05). The expression of VEGF, MMP-9 protein and mRNA in patients with non-invasive pituitary adenoma were significantly lower than those in invasive pituitary adenoma (P <0.05). The levels of VEGF and MMP-9 in patients with invasive pituitary adenomas were not significantly different but were positively correlated with mRNA levels (r = 0.463, P <0.05). Conclusion The results of electron microscopy and immunohistochemistry showed that there was a correlation between ultrastructure and invasive growth in invasive pituitary adenomas, and VEGF and MMP-9 were related to the formation of neovascularization.