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应用RAPD、SSR和SRAP技术,对甘蓝型油菜低芥酸品系APL01与高芥酸品系M083杂交组合的BC1F1群体进行检测,获得251个分子标记,构建了19个连锁群组成的分子标记遗传图谱;应用WinQTLCart2.0对油菜主要脂肪酸组成进行QTL扫描,获得与棕榈酸含量相关的QTL5个,分别位于N3、N8、N10和N13连锁群,其中效应值较大的主效QTLqPA8-1和qPA13分别可解释棕榈酸含量表型变异的11.31%和14.47%。获得与硬脂酸含量相关的QTL3个,分别位于N1、N8和N16连锁群,其中效应值较大的主效QTLqST16可解释硬脂酸含量表型变异的12.22%。获得与油酸含量相关的QTL2个,位于N8和N13连锁群,均为主效QTL,其中qOL8位于N8连锁群的m11e37b~A0226Ba267区间,可解释油酸含量表型变异的11.73%,qOL13位于N13连锁群的m18e46~m20e25a区间,可解释表型变异的27.14%。获得与亚油酸含量相关的QTL3个,其中主效QTLqLI8-1位于N8连锁群,可解释亚油酸含量表型变异的13.25%。获得与亚麻酸含量相关的QTL3个,效应值均较小,属微效QTL。获得与廿碳烯酸含量相关的QTL4个,分别位于N8、N13和N15连锁群,其中主效QTLqEI8-1、qEI8-2和qEI13分别可解释廿碳烯酸含量表型变异的12.20%、10.22%和11.14%。获得与芥酸含量相关的QTL2个,位于N8和N13连锁群,均为主效QTL,其中qER8位于N8连锁群的m11e37b~A0226Ba267区间,可解释芥酸含量表型变异的16.74%;qER13位于N13连锁群的A0301Bb398~m18e46区间,可解释芥酸含量表型变异的31.32%。在N8连锁群的分子标记m11e27b附近及N13连锁群的分子标记m18e46附近存在多个主要脂肪酸的主效QTL,这些标记可用于油菜脂肪酸改良的分子标记辅助选择。
The RAPD, SSR and SRAP techniques were used to detect the BC1F1 population of a combination of low erucic acid line APL01 and high erucic line M083 in Brassica napus. 251 molecular markers were obtained, and 19 linkage group molecular marker genetic maps were constructed QTLs of major fatty acids in rapeseed were obtained by using QTLs of WinQTLCart2.0, and 5 QTLs related to palmitic acid content were obtained, which were located in N3, N8, N10 and N13 linkage groups, respectively. Among them, QTLs qPA8-1 and qPA13 Explain the phenotypic variation of palmitic acid 11.31% and 14.47%. Three QTLs related to stearic acid content were obtained, which were located in linkage groups N1, N8 and N16, respectively. The major QTL qST16 with larger effect value explained 12.22% of phenotypic variation of stearic acid content. Two QTLs related to oleic acid content were obtained, located in the N8 and N13 linkage groups, all of which were major effect QTLs. QOL8 was located in the range of m11e37b ~ A0226Ba267 in the N8 linkage group, accounting for 11.73% of phenotypic variation of oleic acid content. QOL13 was located in N13 The linkage group m18e46 ~ m20e25a interval, explaining 27.14% of phenotypic variation. There were 3 QTLs related to linoleic acid content, of which QTLqLI8-1 was located in N8 linkage group and could explain 13.25% of phenotypic variation of linoleic acid content. There were 3 QTLs related to linolenic acid content, all of which were minor QTLs. Four QTLs related to eicosenoic acid were obtained, which were located in linkage groups N8, N13 and N15, respectively. The major QTLs QEI8-1, qEI8-2 and qEI13 could explain 12.20% and 10.22 of eicosanoid phenotypic variation, respectively % And 11.14%. Two QTLs related to erucic acid content were obtained, located in the N8 and N13 linkage groups, and were major QTLs. Among them, qER8 was located in the range of m11e37b to A0226Ba267 in the N8 linkage group, accounting for 16.74% of the phenotypic variation of erucic acid content. QER13 was located in N13 The linkage group A0301Bb398 ~ m18e46 interval, can explain erucic acid content of phenotypic variation of 31.32%. There are multiple major QTLs for major fatty acids near the molecular marker m11e27b in the N8 linkage group and near the molecular marker m18e46 in the N13 linkage group. These markers can be used for molecular marker-assisted selection of improved oilseed rape fatty acids.